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Contents

[edit] Overview

  • EagleView is an information-rich genome assembler viewer with data integration capability. EagleView can display a dozen different types of information including base qualities, machine specific trace signals, and genome feature annotations. It provides an easy way for inspecting visually the quality of a genome assembly and validating polymorphism candidate sites (e.g., SNPs) reported by polymorphism discovery tools. It can also facilitate data interpretation and hypothesis generation. EagleView is a multi-platform application developed with C++ and is available for all three major platforms: Windows, Linux, and Mac. For more details, please see EagleView publication at EagleView: a genome assembly viewer for next-generation sequencing technologies, Genome Res.,June 11, 2008
  • EagleView reads multiple data files including the standard ACE genome assembly file and optional READS, EGL, and MAP files. These optional files contain additional information of a genome assembly and can be visualized on EagleView. EagleView was designed with user's needs in mind. All these files are in simple text formats, which make it easy for creating customized data files and visualizing user-defined features on EagleView. The detail format for each type file is described in EagleView documentation. When EagleView opens an ACE file, it will automatically detect and load the associated optional files in the same directory.

Key Features

  • fast and memory efficient
  • pin-point view of machine-specific signals pin-point view of base quality
  • pin-point view of read id and strand distinct mark for discrepancy sites
  • genome annotation integration
  • navigation by read id and contig id
  • navigation by genomic features or user defined locations
  • navigation by both unpadded and padded positions
  • data utility tools included
  • zooming and printing capability
  • customizable font and color for viewer

[edit] Software Download

EagleView is distributed separately for different platforms. Each distribution contains everything including the core EagleView software, three utility tools for data preparation, and detailed documentation. The old beta version is still available here.

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[edit] MS Windows (XP or Vista) Distribution

Package: A single binary installer for either 32 or 64-bit system.

Installation: Double click EagleView installer and simply follow its instructions.

Launch: EagleView can launch from either Windows program menu or its shortcut at your Desktop

Download links:

EagleView-2.2-Setup-Win32.exe (32-bit)

EagleView-2.2-Setup-Win64.exe (64-bit)

[edit] Linux Distribution

Package: EagleView is distributed as a single RPM or Debian installation package for both 32-bit and 64-bit Linux systems. RPM packages are available for Fedora Linux (5 or above), Suse Linux (10.2 or above), and Redhat Enterprise Linux (4 or above) platforms. Debian package are available for Debian (4.0 or above) and Ubuntu Linux (7.04 or above). Installation: You need to be the root or a sudo user to install EagleView RPM or Debian package. RPM package Installation is done by issuing one line command at a terminal window:

        sudo rpm -i EagleView-ver.<platform>.rpm

For Upgrade

        sudo rpm -U EagleView-ver.<platform>.rpm

Debian package Installation is done by issuing one line command at a terminal window:

         dpkg -i EagleView-ver.<platform>.deb

Launch: issue the following command at your Linux terminal window:

       eagleview

Download links:

EagleView_2.2_CentOS5_x64_64.tar.gz EagleView tar package for 64-bit CentOS 5 or Redhat Linux 5

EagleView_Linux32.tar.gz(32-bit Linux tar package for Fedora, Suse, and Ubuntu Linux)

EagleView_Linux64.tar.gz(64-bit Linux tar package for Fedora, Suse, and Ubuntu Linux)

EagleView-2.2-0.i386.redhat.rpm(Redhat Enterprise Linux 32-bit)

EagleView-2.2-0.x86_64.redhat.rpm(Redhat Enterprise Linux 64-bit)

EagleView-2.1-0.i386.fedora.rpm(Fedora 32-bit)

EagleView-2.1-0.x86_64.fedora.rpm(Fedora 64-bit)

EagleView-2.1-0.i586.suse.rpm(Suse 32-bit)

EagleView-2.1-0.x86_64.suse.rpm(Suse 64-bit)

eagleview.2.1.i686.ubuntu.deb(Ubuntu Linux 32-bit)

eagleview.2.1.x86_64.ubuntu.deb(Ubuntu Linux 64-bit)

eagleview.2.1.i686.debian.deb (Debian Linux 32-bit)

[edit] Mac (OS X 10.4 or later with Intel CPU) Distribution

Package: A disk image contains Mac OS X installation Package

Installation: You need to have the administrator privileges to install EagleView. Installation is done by double clicking EagleView Installer EagleView_1.5_MacOS_X in the disk image

Launch: double click EagleView application at /Applications/eagleview. The data tools for EagleView should be used in the terminal window.

Download links:

EagleView-2.0.MAC_Installer.dmg (MacOS X with Intel CPU)

[edit] Sample and Test Datasets

[edit] Small Example Datasets

a complete example for 454 read assembly (100KB)

a complete example for Illumina read assembly (2.1MB)

[edit] EagleView MAP files for Human genome annotation and SNP data

dbSNP SNP MAP files (chr1-chr10)(72MB)

dbSNP SNP MAP files (chr11-chrY)(45MB)

dbSNP SNP MAP files HapMap SNP MAP file (CEU population)(36MB)

dbSNP SNP MAP files HapMap SNP MAP file (CHB population)(36MB)

dbSNP SNP MAP files HapMap SNP MAP file (JPT population)(36MB)

dbSNP SNP MAP files HapMap SNP MAP file (YRI population)(36MB)

Org transcript MAP files (171MB)

Zoo transcript MAP files (31MB)

[edit] Datasets for efficiency tests

an e.coli K12 assembly of 6,822,388 Illumina read (199MB)

a c.elegan chromosome II assembly of 14,562,818 Illumina reads (437MB)

a c.elegan chromosome V assembly of 19,566,095 Illumina reads (587MB)

[edit] Contacts

Weichun Huang

Gabor Marth