| Sequence 1KF9F EXTRACELLULAR DOMAIN
HUMAN GROWTH HORMONE
Length 188 residues |
| Cysteines in this sequence:
6 |
|
Disulfide Connectivity prediction |
| Step 1: Running PSI-BLAST with
input sequence |
| Step 2: Predicting
secondary structure using PSIPRED |
| Step
3: Disulfide Oxidation State Prediction |
| Step 4: Disulfide
Bonds Prediction using a trained Neural Network |
| Disulfide bond scores |
| Cysteine
sequence
position | Distance | Bond | Score |
|---|
| 1 -
11 | 10 | PKFTKCRSPER-RETFSCHWTIQ |
0.99544 |
| 1 -
28 | 27 | PKFTKCRSPER-QEWKECPDYVS |
0.03345 |
| 1 -
39 | 38 | PKFTKCRSPER-AGENSCYFNSS |
0.01964 |
| 1 -
53 | 52 | PKFTKCRSPER-IWIPYCIKLTS |
0.0108 |
| 1 -
67 | 66 | PKFTKCRSPER-TVDEKCFSVDE |
0.02249 |
| 11 -
28 | 17 | RETFSCHWTIQ-QEWKECPDYVS |
0.01103 |
| 11 -
39 | 28 | RETFSCHWTIQ-AGENSCYFNSS |
0.01205 |
| 11 -
53 | 42 | RETFSCHWTIQ-IWIPYCIKLTS |
0.01037 |
| 11 -
67 | 56 | RETFSCHWTIQ-TVDEKCFSVDE |
0.01184 |
| 28 -
39 | 11 | QEWKECPDYVS-AGENSCYFNSS |
0.99805 |
| 28 -
53 | 25 | QEWKECPDYVS-IWIPYCIKLTS |
0.01038 |
| 28 -
67 | 39 | QEWKECPDYVS-TVDEKCFSVDE |
0.01049 |
| 39 -
53 | 14 | AGENSCYFNSS-IWIPYCIKLTS |
0.01037 |
| 39 -
67 | 28 | AGENSCYFNSS-TVDEKCFSVDE |
0.01047 |
| 53 -
67 | 14 | IWIPYCIKLTS-TVDEKCFSVDE |
0.99584 |
|
| Step 5: Weighted
matching |
| Predicted bonds |
| 1 - 11 | PKFTKCRSPER - RETFSCHWTIQ |
| 28 - 39 | QEWKECPDYVS - AGENSCYFNSS |
| 53 - 67 | IWIPYCIKLTS - TVDEKCFSVDE |
| Predicted connectivity |
| 1-2, 3-4, 5-6 |
